Figure. Concentration-dependent activation of ADORA1 by NECA. Reporter cells were transfected with either the expression plasmid for adeno­sine A1 receptor (ADORA1) or the mock plasmid and treated with various concentrations of 5'-N-ethyl­carbox­amido­adeno­sine (NECA). Data points shown are the mean ± SEM of an experiment performed in triplicate, and the curve is a fit to Hill equation with an EC50 of 4.8 nM.