Description
Figure. Concentration-dependent activation of ADORA1 by NECA. Reporter cells were transfected with either the expression plasmid for adenosine A1 receptor (ADORA1) or the mock plasmid and treated with various concentrations of 5'-N-ethylcarboxamidoadenosine (NECA). Data points shown are the mean ± SEM of an experiment performed in triplicate, and the curve is a fit to Hill equation with an EC50 of 4.8 nM.