Figure. Concentration-dependent activation of BRS3 by bombesin. Reporter cells were transfected with either the expression plasmid for bombesin receptor subtype 3 (BRS3) or the mock plasmid and treated with various concentrations of bombesin. Data points shown are the mean ± SEM of an experiment performed in triplicate, and the curve is a fit to Hill equation with an EC50 of 2.4 nM.