Figure. Concentration-dependent activation of CASR by cinacalcet. Reporter cells were transfected with either the expression plasmid for calcium sensing receptor (CASR) or the mock plasmid and treated with various concentrations of cinacalcet. Data points shown are the mean ± SEM of an experiment performed in triplicate, and the curve is a fit to Hill equation with an EC50 of 140 nM.