Figure. Concentration-dependent activation of MCHR1 by MCH. Reporter cells were transfected with either the expression plasmid for melanin con­cen­trat­ing hormone receptor 1 (MCHR1) or the mock plasmid and treated with various concentrations of melanin-con­cen­trat­ing hormone (MCH). Data points shown are the mean ± SEM of an experiment performed in triplicate, and the curve is a fit to Hill equation with an EC50 of 37 nM.