Figure. Concentration-dependent activation of NPY2R by NPY. Reporter cells were transfected with either the expression plasmid for neuro­pep­tide Y receptor Y2 (NPY2R) or the mock plasmid and treated with various concentrations of neuro­pep­tide Y (NPY). Data points shown are the mean ± SEM of an experiment performed in triplicate, and the curve is a fit to Hill equation with an EC50 of 4.6 nM.