Figure. Concentration-dependent activation of PRLHR by PrRp-31. Reporter cells were transfected with either the expression plasmid for pro­lactin re­leas­ing hormone receptor (PRLHR) or the mock plasmid and treated with various concentrations of PrRp-31. Data points shown are the mean ± SEM of an experiment performed in triplicate, and the curve is a fit to Hill equation with an EC50 of 1.8 nM.