Figure. Concentration-dependent activation of GPR183 by 7α,25-di­hydroxy­cholesterol. Reporter cells were transfected with either the expression plasmid for G protein-coupled receptor 183 (GPR183) or the mock plasmid and treated with various concentrations of 7α,25-di­hydroxy­cholesterol. Data points shown are the mean ± SEM of an experiment performed in triplicate, and the curve is a fit to Hill equation with an EC50 of 2.0 nM.