Figure. Concentration-dependent activation of ADCYAP1R1 by PACAP. Reporter cells were transfected with either the expression plasmid for ADCYAP receptor type I (ADCYAP1R1) or the mock plasmid and treated with various concentrations of pi­tu­i­tary adenyl­ate cy­clase-acti­vat­ing poly­pep­tide (PACAP). Data points shown are the mean ± SEM of an experiment performed in triplicate, and the curve is a fit to Hill equation with an EC50 of 4.4 nM.