Figure. Concentration-dependent activation of CALCRL by CGRP. Reporter cells were transfected with the expression plasmid for calci­tonin receptor like receptor (CALCRL) and treated with various concentrations of calci­tonin gene-re­lated pep­tide (CGRP). Data points shown are the mean ± SEM of an experiment performed in triplicate, and the curve is a fit to Hill equation with an EC50 of 1.0 nM.