Figure. Concentration-dependent activation of QRFPR by QRFP. Reporter cells were transfected with either the expression plasmid for pyro­glu­tamy­lat­ed RFamide pep­tide receptor (QRFPR) or the mock plasmid and treated with various concentrations of QRFP. Data points shown are the mean ± SEM of an experiment performed in triplicate, and the curve is a fit to Hill equation with an EC50 of 720 pM.