Reproducibility is key when it comes to pharmaceutical research. Tanso Biosciences' GPCR functional assay is not only sensitive and versatile but highly reproducible. We have conducted a series of experiments to verify the consistency of our assay, and the results are impressive.

Experiment 1

We performed concentration-response analyses for β1 adrenoceptor (ADRB1) vs. isoproterenol on three different days (Figure 1, Table 1). Each test yielded a very close EC50 value, indicating excellent reproducibility of the assay.

TestEC50Hill coefficient
33 nM2.8
236 nM2.8
331 nM2.8
Aggregate33 nM2.8
Table 1. Curve-fitting parameters for ADRB1 vs. isoproterenol

Experiment 2

We examined the variability between experimenters. Two experimenters in our lab conducted concentration-response analyses for type 1 angiotensin 2 receptor (AGTR1) and yielded comparable results both in the agonist mode (Figure 2, Table 2) and the antagonist mode (Figure 3, Table 3).

ExperimenterEC50Hill coefficient
1160 pM2.0
2120 pM2.0
Aggregate140 pM2.0
Table 2. Curve-fitting parameters for AGTR1 vs. angiotensin II
ExperimenterIC50Hill coefficient
12.1 nM-1.4
22.4 nM-1.4
Aggregate2.2 nM-1.4
Table 3. Curve-fitting parameters for AGTR1 vs. valsartan


Overall, our verification process demonstrated that Tanso Biosciences' GPCR functional assay consistently produces reliable results, regardless of who conducts the test or when it is conducted. Trust in the reproducibility of our assay and choose Tanso Biosciences for your research needs.